recombinant bacteria meaning in Chinese
重组细菌
Examples
- These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria . the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit , and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128 , 000
从制备性聚丙烯酰胺凝胶中回收融合蛋白，用mtt法测定重组蛋白刺激小鼠脾淋巴母细胞增殖反应活性，并以重组蛋白免疫家兔制备抗猪il - 6滴度为128 ， 000的高免兔血清。 - The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23 . 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )
Rt - pcr分析确证重组质粒在大肠杆菌中能正确转录； sds - page分析证明诱导重组质粒表达了融合蛋白，分子量约为50kd ，与预期的26kd的gst带和23 . 6kd的草鱼生长激素基因编码蛋白质构成的融合蛋白大小一致。 - The mrna and protein expression were assayed by rt - pcr and sds - page . the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )
以iptg诱导融合蛋白表达，经rt - pcr检测发现740bp的特异性il - 6条带；通过sds - page分析，在49kd处出现gst ? pil6融合蛋白条带，其表达量占总细菌蛋白量的30 ，证明猪il - 6基因得到了正确转录和表达。 - The expression conditions of e2 gene in p . pastoris were optimized , the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7 . 5 and 8 . 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e . coli the expression vector pproex - htb respectively , the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e . coli competent bacteria respectively , the recombinant bacteria could express the major antigen region of e2 gene , the expression yields amount to 35 % and 38 % repectively
猪瘟病毒ez基因的原核表达： pcr扩增出当前猪瘟流行野毒株，中国猪瘟兔化弱毒（ c株）兔脾组织毒ez基因的主要抗原区，将其克隆到原核大肠杆菌表达载体pproex htb中诱导表达，经sds page检测表明，重组质粒能表达ez基因主要区蛋白， westernblot检测表明，诱导表达蛋白与猪瘟阳性血清发生特异性反应，表达量为35和38 ，可用于基因工程诊断抗原。